Halldor Felde Berg, | Heidi Cecilie Larsen Spång | Bjørg Heringstad | Erik Ropstad1 | Anne Hege Alm-Kristiansen | Elisabeth Kommisrud

Reproduction in Domestic Animals, Januar 4, 2020

An extended lifespan of spermatozoa following artificial insemination (AI) can make
the timing of insemination less critical, as previously demonstrated with immobilized
spermatozoa that are gradually released from an alginate gel. The purpose was to
examine the in vivo dissolution of SpermVital (SV) alginate gel over time by endoscopy
and secondly to assess spermatozoa quality after incubation of the gel. In vivo
endoscopy showed SV gel in the uterus 3, 6, 20 and 24 hr after AI, demonstrating
the potential release of spermatozoa to the uterus during this period. In utero ex vivo
incubation of the semen demonstrated that high motility and viability of sperm cells
was sustained following overnight incubation.